Journal: Molecular cancer research : MCR

Article Title: Caspase-9b drives cellular transformation, lung inflammation, and lung tumorigenesis

doi: 10.1158/1541-7786.MCR-21-0905

Figure Lengend Snippet: Macroscopic (A) and microscopic lung tumors observed in C9b mice over 21m old of age are summarized in B. *: One mouse developed a carcinoid tumor and adenocarcinoma. C. Histological analyses including H&E (a-c), Alcian Blue-PAS (d), and IHC staining with indicated antibodies (e-i) of lung tumors identified adenocarcinoma (a,d, g-i), squamous cell carcinoma (scc, b&e), and carcinoid tumors (c&f). Images are taken at 10x magnification. Inset in b is at 40x magnification. D&E. Gr1+ cells (D, IHC) in the lung per low power field (20x, 3 views per mouse) were counted in control (n=9) and C9b mice with (red, n= 5) or without (n=11) lung tumors (E). Data are means ± SEM. Adjusted p values are determined by ANOVA with Tukey’s multiple comparison test.

Article Snippet: Gomori Trichrome staining (Richard-Allan Scientific) and Alcian blue-PAS staining (Statlab) were performed following manufacturers’ instructions.

Techniques: Immunohistochemistry, Control, Comparison

Journal: Bioactive Materials

Article Title: Mesenchymal stem cell activity across a graded scaffold-hydrogel composite biomaterial for tendon-to-bone enthesis repair

doi: 10.1016/j.bioactmat.2025.07.017

Figure Lengend Snippet: Histological evaluation of triphasic scaffold structure and matrix remodeling. FFPE sections of hMSC-seeded triphasic scaffolds (n = 2) at both Day 1 and Day 21 were stained with either trichrome, alizarin red, or alcian blue staining. Representative images are provided of (A) a scan of a full scaffold (trichrome only), (B) the osseous region, (C) the tendinous region, and (D) the interfacial region.

Article Snippet: Samples were stained by one of four staining procedures: hematoxylin/eosin (H&E, SL200 & SL201, StatLab), alizarin red (Cat# 2003999, EMD Millipore Corp.), trichrome (Cat# ab150686, Abcam), or alcian blue (pH 2.5, Cat# KTABP2.5, StatLab) with staining procedures followed according to manufacturer recommendation.

Techniques: Staining

Journal: Scientific Reports

Article Title: Electrical stimulation drives chondrogenesis of mesenchymal stem cells in the absence of exogenous growth factors

doi: 10.1038/srep39302

Figure Lengend Snippet: ( a ) Immunofluorescent staining (COL2A1; green) and alcian blue staining (glycosaminoglycan (GAG); blue) of micromass cultures after culture in maintenance medium (control) or maintenance medium with ES (5 V/cm, 8 ms, 5 Hz) for 3 days (ES). Nuclei were stained blue using Hoechst 33342; Scale bars, 500 μm. ( b ) Immunofluorescent staining (COL2A1; green) and alcian blue staining (GAG; blue) of paraffin-embedded sections of micromass cultures after culture in maintenance medium (control) or maintenance medium with ES (5 V/cm, 8 ms, 5 Hz) for 3 days (ES). Nuclei were stained blue using Hoechst 33342; Scale bars, 100 μm. (c) Quantitative analysis of immunostaining intensity and alcian blue staining intensity. Data are presented as means ± S.D. and differences were identified using Students t-test, **p < 0.01.

Article Snippet: To visualize accumulation of sulfated glycosaminoglycans (GAGs), cells were rinsed with PBS, fixed in paraformaldehyde for 20 min, stained with Alcian Blue Solution (pH 2.5; Nacalai tesque, INC, Japan) overnight at room temperature, and were then rinsed with distilled water three times.

Techniques: Staining, Control, Immunostaining

Journal: Gastroenterology

Article Title: Kr?ppel-like Factor 5 is Important for Maintenance of Crypt Architecture and Barrier Function in Mouse Intestine

doi: 10.1053/j.gastro.2011.06.086

Figure Lengend Snippet: Loss of a restricted differentiation zone and changes in epithelial cell lineage with deletion of Klf5. Paraffin-embedded colon sections from adult control and Klf5 mutant mice were stained with various epithelial markers to identify changes in differentiation. (A) Immunohistochemical staining with the colonic differentiation marker, carbonic anhydrase-I. (B) Quantitative real time polymerase chain reaction (qRT-PCR) to measure relative mRNA expression of Cdx1 and Cdx2 in colon tissues from control and mutant mice (*P < 0.001, **P = 0.004, n=3). (C) Alcian blue/Periodic acid-Schiff (PAS) staining to identify goblet cells and immunohistochemical staining of chromogranin A as a marker for enteroendocrine cells.

Article Snippet: Alcian blue/PAS staining of paraffin-embedded sections to identify goblet cells was carried out using the Alcian Blue and PAS Staining Kit (Biocare Medical, Concord, CA) per manufacturer's instructions.

Techniques: Control, Mutagenesis, Staining, Immunohistochemical staining, Marker, Real-time Polymerase Chain Reaction, Quantitative RT-PCR, Expressing